Selected staining methods for plant tissue
In plant tissues stained with this method cell walls stain blueblack, nuclei stain yellow to orange, starch grains appear black, and lignified cell walls stain brilliant red (Foster, 1934; Sharman, 1943). ... Wash in DI for 5–15 s. Dehydrate through 45, 90, EtOH, about 10 s each step.